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The Elizabeth plant opened in 1963 and at its peak, between June 2003 and July 2005, produced 780 vehicles per day, including the VY Commodore, the Adventra, Crewman, Caprice and Statesman. Holden enthusiasts have lined the street outside the Elizabeth factory.
Shilane Quist brought her Holden Torana to show support for the workers and their families."I'm just proud that Elizabeth has got something like this, and it's very sad it'll be closing today," she said.
It's just really sad that it has to all end." Holden said the decommissioning of the factory was expected to take about 18 months.
It achieved its largest market share of national sales at 50.3 per cent in 1958.
Elizabeth was home to Australia's last road-vehicle manufacturing plant after Toyota closed its Camry factory at Altona, Victoria, earlier this month.
There is archaeological evidence that javelins and throwing sticks were already in use by the last phase of the lower Paleolithic.
Seven spear-like objects were found in a coal mine in the city of Schöningen, Germany.